Stanford Scientists Use Copy/Paste Method to Make Cells HIV Resistant
January 24, 2013 8:59 AM
comment(s) - last by
The method stopped HIV from entering healthy immune cells
Stanford scientists have found a way to protect the immune system from HIV by
placing resistant genes into T cells
Researchers from the Stanford University School of Medicine, led by Matthew Porteus, MD, have used a cut and paste method where HIV-resistant genes were coupled with T cells to deny the virus' entry into healthy immune cells.
The HIV virus typically enters immune cells by binding to one of two surface proteins: CCR5 and CXCR4. However, some people have a mutation in CCR5 that makes them resistant to HIV.
Porteus and his team used this idea to create a method for making this protein inactive. They used a protein, called a zinc finger nuclease, that finds and attaches to the CCR5 receptor gene and modifies it to imitate the mutated, inactive versions. It does this by breaking up pieces of DNA.
In addition to breaking a sequence in the CCR5 receptor's DNA, the team pasted three genes that are resistant to HIV. They help protect the cells via both the CCR5 and CXCR4 receptors. This technique is called stacking, where multiple layers of protection are used to protect the cells.
In tests, T cells with single, double and triple gene modifications were protected against HIV. However, as expected, the triplets were much more resistant to infection. In fact, they had 1,200-fold
protection against HIV
carrying the CCR5 receptor and 1,700-fold protection against those carrying the CXCR4 receptor.
T cells without any protection were infected within 25 days.
"We inactivated one of the receptors that HIV uses to gain entry and added new genes to protect against HIV, so we have multiple layers of protection -- what we call stacking," said Porteus. "We can use this strategy to make cells that are resistant to both major types of HIV."
There are two issues that the team has to work out, though. First, the zinc finger nuclease could cause a break elsewhere in the DNA and cause cancer. Second, the cells may not accept the genetic change.
Stanford School of Medicine
This article is over a month old, voting and posting comments is disabled
RE: Target demograph
1/24/2013 3:34:16 PM
1983 called, they want their idiocy back.
"If you look at the last five years, if you look at what major innovations have occurred in computing technology, every single one of them came from AMD. Not a single innovation came from Intel." -- AMD CEO Hector Ruiz in 2007
FDA Approves New "OraQuick" HIV Home Test Kit
July 5, 2012, 9:42 AM
New Technique Renders T-Cells Resistant to HIV
January 27, 2011, 8:51 AM
Texas Chosen as Site for SpaceX's First Commercial Launchpad
August 5, 2014, 1:44 PM
South Carolina Prison Finds Crashed Drone Carrying Drugs, Phones
August 1, 2014, 2:49 PM
NASA's Mars 2020 Rover Gains Seven New Instruments for Exploration
August 1, 2014, 1:30 PM
NASA Opportunity Rover Breaks Record for Most Miles Traveled on Another Planet
July 29, 2014, 1:38 PM
Commercial Drones to Get Privacy Guidelines Via Executive Order
July 25, 2014, 5:34 PM
Pentagon's Priciest Project, F-35, Misses International Debut
July 25, 2014, 10:18 AM
Most Popular Articles
Lumia 830 Gets Major Upgrades Including New 20.1 Megapixel Toshiba Sensor
August 15, 2014, 6:00 PM
Windows Phone, BlackBerry Smartphone Market Share Falls to 2.5%, 0.5% Respectively
August 15, 2014, 9:44 AM
GM Concedes That the Cadillac ELR Doesn’t Really Compete with the Tesla Model S
August 15, 2014, 5:42 PM
Report: Windows 9 “Threshold” Tech Preview Coming Next Month
August 15, 2014, 11:29 AM
Apple Scores Patents, Preps New Reversible USB Plug for iPhone 6 Connector
August 18, 2014, 1:32 PM
Latest Blog Posts
Space Terrorism is a Looming Threat For the United States
Apr 23, 2014, 7:47 PM
Facebook Aims to Provide Internet to "Every Person in the World" with Drones, Satellites
Apr 1, 2014, 10:20 AM
Retail Mobile Sites Experience Outages in Light of Simplexity's Bankruptcy
Mar 14, 2014, 8:48 AM
Tesla vs. BMW: Who Has the Safer EV?
Feb 1, 2014, 2:56 PM
Justice Leaks Details of Next HTC One Two Flagship Phone
Dec 5, 2013, 4:04 PM
More Blog Posts
Copyright 2014 DailyTech LLC. -
Terms, Conditions & Privacy Information