Improved Technique Produces Array of Human-made DNA Sequences
January 26, 2011 12:43 PM
comment(s) - last by
(Source: Duke University Photography )
New faster technique could lead to the development of new types of drugs
have created an improved technique that is capable of producing nearly an unlimited array of human-made DNA sequences quickly and easily.
Ashutosh Chilkoti, study leader and Theo Pilkington Professor of Biomedical Engineering at Duke University's Pratt School of Engineering, along with Daniel Callahan, of Duke University, and graduate students Miriam Amiram and García Quiroz, have developed a method for creating human-made DNA sequences faster in order to produce repetitive proteins, which are used to make new bioengineered tissues and drugs.
Traditional techniques for creating DNA sequences are not robust or very slow, which has prevented the development of new classes of protein-based polymers.
But now, the Duke University researchers have created
a new process
called overlap-extension rolling circle amplification. The process attaches a macromolecule, or polymer, to a protein, which allows the protein to maintain activity in the bloodstream longer and increases effectiveness. But instead of building these protein building blocks individually the way traditional methods do now, this new technique can make almost an unlimited number of them.
"Depending on how complicated you want the polymer sequence to be, there are an infinite number of combinations you could make," said Chilkoti. "We haven't even begun to look at all the sequences that can be made or the unique properties they might have."
One beneficial trait to this new technique is that it is an improvement to already-existing methods, which makes it inexpensive to use in the laboratory.
"A very popular method for making tandem copies of
involves inserting them iteratively into a bacterial plasmid," said Amiram. "After the vector has grown in size, the copies of the sequence are cut out using enzymes and the process is repeated to generate a larger polymer. It is a very time-consuming process.
"With this new method, you don't get just one product, but many. This should help us to make large libraries of proteins, which we can use to rapidly screen new combinations. This powerful strategy generates
libraries of repetitive genes
over a wide range of molecular weights in a 'one-pot' parallel format."
To test this improved technique, the Duke University researchers synthesized genes located in two different classes of protein-polymers. The first was protein-polymer combinations for elastin, which is found in connective tissue. The second synthesized genes in glucagon-like peptide-1 analogs "to show variable pharmokinetic properties. Researchers were successfully able to synthesize genes in both cases.
"This could help remove one of the biggest stumbling blocks we face in producing these drugs," said Chilkoti. "You can't make the proteins without genes, which act as the software directing the protein's production. Instead of building each sequence individually, as is done now, we can literally make hundreds, each with subtle differences."
was published in
This article is over a month old, voting and posting comments is disabled
1/30/2011 10:13:23 AM
Natural DNA can't fight cancer or AIDS.
"It's okay. The scenarios aren't that clear. But it's good looking. [Steve Jobs] does good design, and [the iPad] is absolutely a good example of that." -- Bill Gates on the Apple iPad
"Tunneling Electrode Junction" Device Could Sequence Human DNA in Minutes
December 21, 2010, 10:05 AM
First Family Genome Sequenced, Offers Insight on Disease
March 11, 2010, 1:28 PM
Australian Engineers Successfully Developed 3D-Printed Jet Engines
March 2, 2015, 11:08 AM
Smartphone STD Scanner Dongle Can Detect HIV in Just 15 Minutes
February 26, 2015, 11:04 AM
Bill Gates Plugs GMOs to Reduce Starvation in Africa
February 24, 2015, 10:12 PM
Cool Nature Video: Crazy Coconut Octopus Braves Dry Land to Capture Crab
February 24, 2015, 4:18 PM
MIT Creates Injectable Self-Healing Nanoparticle Hydrogel for Cancer Treatment
February 23, 2015, 9:37 AM
Amp EVs' Workhorse and HorseFly Duo Challenge Amazon to Delivery Drone Duel
February 20, 2015, 3:56 AM
Most Popular Articles
Google Steps up Snub of Adobe Flash, Auto-Converting Flash Ads to HTML5
February 25, 2015, 6:16 PM
FCC Bans Data Discrimination, Defies Comcast, Adopting Net Neutrality Regulation
February 26, 2015, 4:03 PM
Windows 10 Adds USB 3.1 for Dual-Role Peripherals, External Display Support
February 27, 2015, 11:39 AM
StarDock Unveils Start10 Start Menu Replacement for Windows 10
February 25, 2015, 11:24 AM
Google Preps Pixel 2 ChromeBook, New X Server Replacement "Freon"
February 24, 2015, 11:12 PM
Latest Blog Posts
Sceptre Airs 27", 120 Hz. 1080p Monitor/HDTV w/ 5 ms Response Time for $220
Dec 3, 2014, 10:32 PM
Costco Gives Employees Thanksgiving Off; Wal-Mart Leads "Black Thursday" Charge
Oct 29, 2014, 9:57 PM
"Bear Selfies" Fad Could Turn Deadly, Warn Nevada Wildlife Officials
Oct 28, 2014, 12:00 PM
The Surface Mini That Was Never Released Gets "Hands On" Treatment
Sep 26, 2014, 8:22 AM
ISIS Imposes Ban on Teaching Evolution in Iraq
Sep 17, 2014, 5:22 PM
More Blog Posts
Copyright 2015 DailyTech LLC. -
Terms, Conditions & Privacy Information