Muscle Injury in Mice Repaired with Stem Cell Transplant
November 12, 2010 8:57 AM
comment(s) - last by
Mice with muscle injuries obtained increased muscle mass with stem cell transplant
Researchers from the
University of Colorado at Boulder
have found that loss of muscle function and mass, which is a result of aging, can be prevented by
transplanting certain types of stem
into the legs of mice.
Bradley Olwin, study leader and professor of CU-Boulder's molecular, cellular and developmental biology department, along with co-authors John K. Hall, Glen Banks and Jeffrey Chamberlain, have discovered that mice with limb muscle injuries can be repaired by injecting muscle stem cells from donor mice into the leg muscle.
Olwin and his team made the discovery by drawing between 10 and 50 stem cells as well as attached myofibers, which are individual skeletal muscles, from healthy three-month old mice, and transplanted the
into three-month-old injured mice. These muscle stem cells were found in populations of satellite cells, which repair and maintain skeletal muscles and are located between muscle fibers and nearby connective tissue.
Only days after the injection, the injury was repaired. In addition, the treated muscle increased 50 percent in mass with a 170 percent increase in size, and was able to sustain itself throughout the rest of the mouse's life, which was about two years.
"We found that the transplanted stem cells are permanently altered and
reducing the aging
of the transplanted muscle, maintaining strength and mass," said Olwin.
Researchers expected the transplanted cells to be injected into the injured muscle, repopulate, repair the muscle and dissipate. But they were shocked when the cells constantly maintained the repaired muscle.
"In this study, the hallmarks we see with the aging of muscles just weren't occurring," said Olwin. "The transplanted material seemed to kick the stem cells to a high gear for self-renewal, essentially taking over the production of muscle cells. But the team found that when transplanted stem cells and associated myofibers were injected to healthy mouse limbs muscles, there was no discernible evidence for muscle mass growth.
"The environment that the stem cells are injected into is very important, because when it tells the cells there is an injury, they respond in a unique way. We don't yet know why the cells we transplanted are not responding to the environment around them in the way that the cells that are already there respond. It's fascinating, and something we need to understand."
Researchers flagged donor cells in injured mice by using green
under ultraviolet light. They found that several transplanted cells were fused to myofibers and that the number of satellite cells had increased.
"It's our hope that we can someday identify small molecules or combinations of small molecules that could be applied to endogenous muscle stem cells of humans to mimic the behavior of transplanted cells," said Olwin. "This would remove the need for cell transplants altogether, reducing the risk and complexity of treatments."
was published in
Science Translational Medicine
on November 10.
This article is over a month old, voting and posting comments is disabled
11/12/2010 1:49:19 PM
That, and bruised up meat tastes like crap.
"Vista runs on Atom ... It's just no one uses it". -- Intel CEO Paul Otellini
Researchers Directly Convert Human Skin Cells into Blood Stem Cells
November 8, 2010, 12:52 PM
New Ageless, More Elastic Stem Cells Developed
October 4, 2010, 11:37 AM
Damaged Hearts Repaired With "Glowing" Firefly Stem Cells
September 29, 2010, 1:39 PM
New Laser Treatment Could Make Incandescent Bulbs as Efficient as Fluorescent
June 2, 2009, 7:23 AM
Creationists are Mad About Google Doodle Depicting Evolution
November 24, 2015, 8:48 PM
DHS and TSA: Whoops, We Missed That 73 Airport Employees May be Terrorists
November 19, 2015, 2:16 PM
Star Wars Spinoff Film "Rogue One", Theme Park Attractions Announced
August 17, 2015, 12:20 PM
SpaceX Falcon 9's Seventh Supply Mission to ISS Ends w/ Fiery Stage 1 Explosion
June 28, 2015, 1:10 PM
Cool Science Video: Glowing Millipede Prowls the Nevada Desert
May 18, 2015, 12:00 PM
Newly Discovered Costa Rican Glass Frog is Kermit's Doppelgänger
April 22, 2015, 11:26 AM
Latest Blog Posts
Sceptre Airs 27", 120 Hz. 1080p Monitor/HDTV w/ 5 ms Response Time for $220
Dec 3, 2014, 10:32 PM
Costco Gives Employees Thanksgiving Off; Wal-Mart Leads "Black Thursday" Charge
Oct 29, 2014, 9:57 PM
"Bear Selfies" Fad Could Turn Deadly, Warn Nevada Wildlife Officials
Oct 28, 2014, 12:00 PM
The Surface Mini That Was Never Released Gets "Hands On" Treatment
Sep 26, 2014, 8:22 AM
ISIS Imposes Ban on Teaching Evolution in Iraq
Sep 17, 2014, 5:22 PM
More Blog Posts
Copyright 2016 DailyTech LLC. -
Terms, Conditions & Privacy Information