Muscle Injury in Mice Repaired with Stem Cell Transplant
November 12, 2010 8:57 AM
comment(s) - last by
Mice with muscle injuries obtained increased muscle mass with stem cell transplant
Researchers from the
University of Colorado at Boulder
have found that loss of muscle function and mass, which is a result of aging, can be prevented by
transplanting certain types of stem
into the legs of mice.
Bradley Olwin, study leader and professor of CU-Boulder's molecular, cellular and developmental biology department, along with co-authors John K. Hall, Glen Banks and Jeffrey Chamberlain, have discovered that mice with limb muscle injuries can be repaired by injecting muscle stem cells from donor mice into the leg muscle.
Olwin and his team made the discovery by drawing between 10 and 50 stem cells as well as attached myofibers, which are individual skeletal muscles, from healthy three-month old mice, and transplanted the
into three-month-old injured mice. These muscle stem cells were found in populations of satellite cells, which repair and maintain skeletal muscles and are located between muscle fibers and nearby connective tissue.
Only days after the injection, the injury was repaired. In addition, the treated muscle increased 50 percent in mass with a 170 percent increase in size, and was able to sustain itself throughout the rest of the mouse's life, which was about two years.
"We found that the transplanted stem cells are permanently altered and
reducing the aging
of the transplanted muscle, maintaining strength and mass," said Olwin.
Researchers expected the transplanted cells to be injected into the injured muscle, repopulate, repair the muscle and dissipate. But they were shocked when the cells constantly maintained the repaired muscle.
"In this study, the hallmarks we see with the aging of muscles just weren't occurring," said Olwin. "The transplanted material seemed to kick the stem cells to a high gear for self-renewal, essentially taking over the production of muscle cells. But the team found that when transplanted stem cells and associated myofibers were injected to healthy mouse limbs muscles, there was no discernible evidence for muscle mass growth.
"The environment that the stem cells are injected into is very important, because when it tells the cells there is an injury, they respond in a unique way. We don't yet know why the cells we transplanted are not responding to the environment around them in the way that the cells that are already there respond. It's fascinating, and something we need to understand."
Researchers flagged donor cells in injured mice by using green
under ultraviolet light. They found that several transplanted cells were fused to myofibers and that the number of satellite cells had increased.
"It's our hope that we can someday identify small molecules or combinations of small molecules that could be applied to endogenous muscle stem cells of humans to mimic the behavior of transplanted cells," said Olwin. "This would remove the need for cell transplants altogether, reducing the risk and complexity of treatments."
was published in
Science Translational Medicine
on November 10.
This article is over a month old, voting and posting comments is disabled
11/12/2010 1:49:19 PM
That, and bruised up meat tastes like crap.
"So if you want to save the planet, feel free to drive your Hummer. Just avoid the drive thru line at McDonalds." -- Michael Asher
Researchers Directly Convert Human Skin Cells into Blood Stem Cells
November 8, 2010, 12:52 PM
New Ageless, More Elastic Stem Cells Developed
October 4, 2010, 11:37 AM
Damaged Hearts Repaired With "Glowing" Firefly Stem Cells
September 29, 2010, 1:39 PM
New Laser Treatment Could Make Incandescent Bulbs as Efficient as Fluorescent
June 2, 2009, 7:23 AM
Crowdfunded Sex Toy Lets You Control the Action From Your Wii Nunchuk
January 15, 2015, 3:42 PM
Elon Musk Plays Warcraft; But Still Finds Time to Sleep, Build a Mars Colony Ship
January 6, 2015, 9:54 PM
SpaceX Scrubs Falcon 9 Launch to ISS, Barge Landing Test, Friday Redo Targeted
January 6, 2015, 4:02 PM
Quick Note; Wreckage of Malaysian-Owned Passenger Jet Found
December 30, 2014, 12:42 PM
Quick Note: Drone Loses Fight to the Death With Kangaroo
December 23, 2014, 3:28 PM
Campbell's Monkeys Found to Use Different Dialects to Describe Local Threats
December 22, 2014, 3:52 PM
Most Popular Articles
Microsoft Shows Off Latest Windows 10 Build, Preps it for Next Week Release
January 21, 2015, 2:57 PM
Under the Hood: How DirectX 11.3 and 12 Will Supercharge Windows 10 Gaming
January 23, 2015, 12:34 PM
BlackBerry CEO Claims Devs are Violating Net Neutrality by Not Supporting BB10
January 22, 2015, 4:37 PM
Report: Samsung Drops Snapdragon 810 From Galaxy S6, Will Use Its Own Chips
January 21, 2015, 1:47 PM
Will Google Become America's Fifth Major Carrier?
January 22, 2015, 12:42 PM
Latest Blog Posts
Sceptre Airs 27", 120 Hz. 1080p Monitor/HDTV w/ 5 ms Response Time for $220
Dec 3, 2014, 10:32 PM
Costco Gives Employees Thanksgiving Off; Wal-Mart Leads "Black Thursday" Charge
Oct 29, 2014, 9:57 PM
"Bear Selfies" Fad Could Turn Deadly, Warn Nevada Wildlife Officials
Oct 28, 2014, 12:00 PM
The Surface Mini That Was Never Released Gets "Hands On" Treatment
Sep 26, 2014, 8:22 AM
ISIS Imposes Ban on Teaching Evolution in Iraq
Sep 17, 2014, 5:22 PM
More Blog Posts
Copyright 2015 DailyTech LLC. -
Terms, Conditions & Privacy Information