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HIV virus  (Source:
The method stopped HIV from entering healthy immune cells

Stanford scientists have found a way to protect the immune system from HIV by placing resistant genes into T cells.

Researchers from the Stanford University School of Medicine, led by Matthew Porteus, MD, have used a cut and paste method where HIV-resistant genes were coupled with T cells to deny the virus' entry into healthy immune cells.

The HIV virus typically enters immune cells by binding to one of two surface proteins: CCR5 and CXCR4. However, some people have a mutation in CCR5 that makes them resistant to HIV.

Porteus and his team used this idea to create a method for making this protein inactive. They used a protein, called a zinc finger nuclease, that finds and attaches to the CCR5 receptor gene and modifies it to imitate the mutated, inactive versions. It does this by breaking up pieces of DNA.

In addition to breaking a sequence in the CCR5 receptor's DNA, the team pasted three genes that are resistant to HIV. They help protect the cells via both the CCR5 and CXCR4 receptors. This technique is called stacking, where multiple layers of protection are used to protect the cells.

In tests, T cells with single, double and triple gene modifications were protected against HIV. However, as expected, the triplets were much more resistant to infection. In fact, they had 1,200-fold protection against HIV carrying the CCR5 receptor and 1,700-fold protection against those carrying the CXCR4 receptor.

T cells without any protection were infected within 25 days.

"We inactivated one of the receptors that HIV uses to gain entry and added new genes to protect against HIV, so we have multiple layers of protection -- what we call stacking," said Porteus. "We can use this strategy to make cells that are resistant to both major types of HIV."

There are two issues that the team has to work out, though. First, the zinc finger nuclease could cause a break elsewhere in the DNA and cause cancer. Second, the cells may not accept the genetic change.

Sources: Science Daily, Stanford School of Medicine

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The waiting game.
By drycrust3 on 1/24/2013 10:12:09 AM , Rating: 3
In fact, they had 1,200-fold protection against HIV carrying the CCR5 receptor and 1,700-fold protection against those carrying the CXCR4 receptor.

It seems to me that if the HIV virus has mutated so one strain can infect people with the CCR5 receptor while another strain infects people with the CXCR4 receptor, then how long will it be before it mutates to produce a strain that infects people with the modified receptors?
There are lots of other questions that need to be asked, but two that do stand out are: 1) can this help a person who is already infected? and 2) will this be passed on to the medicated person's children?

RE: The waiting game.
By TheDoc9 on 1/24/2013 12:24:50 PM , Rating: 2
There are a million issues that can come from messing with genetics. For example they said in the article that the treatment could cause cancer. If the risk was low enough it could be worth it, either way it will likely require decades of testing.

RE: The waiting game.
By drycrust3 on 1/25/2013 12:21:20 PM , Rating: 2
There are a million issues that can come from messing with genetics.

Yes there are, but for me one issue that stood out regarding this was would this create a "different" human genome from what we currently have. About 99% of the human genome is common to all humans, and the thought was that this might suddenly pollute the gene pool with something that has long term consequences.

By AntDX316 on 1/24/13, Rating: 0
Target demograph
By chµck on 1/24/13, Rating: -1
RE: Target demograph
By Obujuwami on 1/24/2013 11:23:30 AM , Rating: 5
Yeah, cuz only homosexuals have HIV, right? No heterosexuals, heroin junkies, diabetics, or blood donors have HIV or AIDS? Africa is infected with HIV and AIDS cuz they are all homosexuals...seems legit!


RE: Target demograph
By ClownPuncher on 1/24/2013 3:34:16 PM , Rating: 5
1983 called, they want their idiocy back.

RE: Target demograph
By Azethoth on 1/28/2013 2:22:04 PM , Rating: 1
Do you gay bash because you are uncomfortable about secretly liking men?

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